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KMID : 0613820080180101331
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Journal of Life Science
2008 Volume.18 No. 10 p.1331 ~ p.1335
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Isolation and Characterization of Klebsiella pneumoniae WL-5 Capable of Decolorizing Triphenylmethane and Azo Dyes
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Wu Jing
Lee Young-Choon
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Abstract
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A Klebsiella pneumoniae WL-5 with the capability of decolorizing several recalcitrant dyes was isolated from activated sludge of an effluent treatment plant of a textile and dyeing industry. This strain showed a higher dye decolorization under static condition and color removal was optimal at pH 6-8 and 30-35oC. More than 90% of its color of Congo Red were reduced within 12 hr at 200 μM dye concentration. Malachite Green, Brilliant Green and Reactive Black-5 lost over 85% of their colors at 10 μM dye concentration, but the percentage decolorization of Reactive Red-120, Reactive Orange-16, and Crystal Violet were about 46%, 25%, and 13%, respectively. Decolorizations of Congo Red and triphenylmethane dyes, such as Malachite Green, Brilliant Green, and Crystal Violet were mainly due to adsorption to cells, whereas azo dyes, such as Reactive Black-5, Reactive Red-120, and Reactive Orange-16 seemed to be removed by biodegradation through unknown enzymatic processes.
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KEYWORD
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Decolorization, Klebsiella pneumoniae, azo dye, triphenylmethane dye
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